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Methoden

imaging methods

List of imaging methods offered in BioSupraMol and of according instruments available from OpenIRIS providers and working groups.

BioSupraMol_200x109_300r

Optical microscopy

Methods:

  • Transmission microscopy
  • Fluorescence microscopy
  • DIC - Differential Interference Contrast Microscopy
  • Histological Imaging with colour camera
  • Living cell microscopy
  • Multi channel imaging
  • Mosaic imaging
  • Phase contrast microscopy
  • Polarisation microscopy
  • Time series with temperature and CO2 control
  • Z stacks

Devices:

  • Zeiss Axioskop 40 POL ( BA-Unit, AG Haag)
  • Zeiss Axio Observer 7 ApoTome.2 ( BA-Unit, SupraFAB)
  • Zeiss Axio Observer.Z1 (ColorCam) ( BA-Unit, AG Haag)
  • Zeiss Axio Observer.Z1 (Incubator) ( BA-Unit, BSM)
  • Zeiss Axio vert.A1 (Fluo) ( BA-Unit, SupraFAB)
  • Zeiss PrimoVert ( MF-Unit, SupraFAB)

High resolution microscopy

Methods:

  • CLSM - Confocal Laser Scanning Microscopy
  • DIC - Differential Interference Contrast Microscopy
  • FRAP - Fluorescence Recovery After Photobleaching
  • FRET - Förster-Resonance Energy Transfer
  • iSCAT - Interferometric scattering microscopy
  • MPM - Multi Photon Microscopy
  • SMLM - Single Molecule Localisation Microscopy
  • STED - STimulated Emission Depletion
  • STORM - STochastic Optical Reconstruction Microscopy
  • Structured illumination
  • TIRF - Total Internal Reflection Fluorescence

Devices:

  • Abberior STED Expert Line ( OM-Unit, SupraFAB)
  • Bruker Vutara 532 (AG Ewers)
  • Leica Multiphotonenmikroskop (AG Hiesinger)
  • Leica SP8 (1) CLSM, S1 ( OM-Unit, BSM)
  • Leica SP8 (2) CLSM ( OM-Unit, BSM)
  • Nikon Ti-E Eclipse (AG Block)
  • Refyne One MP iSCAT ( Analytics-Lab, SupraFAB)
  • Zeiss Axio Observer 7 ApoTome.2 ( BA-Unit, SupraFAB)
  • Zeiss Axio Observer.Z1 (Incubator) ( BA-Unit, SupraFAB)

Electron microscopy

Methods:

  • Transmission electron microscopy (TEM)
  • cTEM - conventional TEM
  • cryoTEM - cryogenic TEM
  • EPU/SPA - automated data acquisition
  • eTOMO - electron tomography
  • STEM - Scanning transmission electron microscopy
  • microED - Micro electron diffraction
  • SEM - Scanning electron microscopy

 Devices:

X-ray structure analysis

Methods:

  • Powder diffraction
  • Single crystal diffraction

Devices:

  • Bruker D8 Venture SC-XRD ( XRD-Unit, BSM)
  • Bruker D8 Venture SC-XRD ( XRD-Unit, BSM)
  • Malvern Panalytical Empyrean (Geo-Unit, BSM)

Surface Imaging

Methods:

  • AFM/SFM - Atomic Force Microscopy
  • SEM - Scaning Electron Microscopy
  • TOF-SIMS - Time Of Flight Secondary Ion Mass Specrometry
  • EPMA - Electron Probe Micro Analysis

Devices:

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