With the ESI-IMS-MS/MS, an UPLC-Synapt G2-S HDMS by Waters Co., a new instrument has arrived at the mass spectrometry core facility.
The system consists of two instruments coupled together:
An extremely powerful chromatographic system, an Acquity UPLC
This machine consists of a binary pump system which allows generating pressures up to 1000 bar (15000 psi). With these pumps, chromatographic columns packed with extremely small particles (down to 1.7 µm) can be used. Very efficient and extremely fast separations can be performed.
Further more it consists of an autosampler which can be cooled down to 4°C, a column oven, and a photodiode array detector (PDA), which is capable of detecting light in the visible and the UV range.
Since capillaries with an extremely small inner diameter and tiny particles as packing material are used, only specially filtered eluents and samples can be used to prevent blockage.
An ESI-IMS-MS/MS (Synapt G2-S HDMS).
The hybrid mass spectrometer principally consists of a Q-TOF instrument with the option to perform ion mobility measurements as well.As options for ionization we currently have either a Z-Spray-ESI-ion source or a NSI-ion source. Both are equipped with a second sprayer (lock spray). This allows additional external calibration while measuring. Thus, an extremely good mass accuracy can be achieved (about 1 to 2 ppm). The resolution of this Time-of-Flight (TOF) mass spectrometer is very high as well - more than 40.000 (FWHM) can be achieved. The extremely high mass range (m/z > 30.000) is very interesting, especially for very large, non-covalently bound complexes like intact antibodies. A further benefit of this instrument is its ion mobility cell. The separation in this region of the instrument is not only induced by the m/z of the ions analyzed, but also by their shape. Thus, not only a very fast separation of very complex mixtures is possible, but also the separation of isobaric substances like peptides with reverse sequences. The IMS is also very important, because the collision cross sections of the ions can be derived from the drift times.
When ESI or NSI is used, the mass spectra will show cationized ions, [M+H]+ or [M+(Catn+)]n+, as well as multiply charged ions. Even for non-covalent complexes, fragment ions are rare. Since the instrument is very sensitive, chemical noise will add up to the background spectrum. The basic requirements which are pointed out in the FTICR-MS and HPLC-MS sections are valid for this instrument as well.
Measurements carried out by the service personnel are offered. The instrument also can be used for long-term research projects carried out by a limited number of specially trained users.
The UPLC and the HPLC system are both equipped with an additional DAD detector. Within the service we offer a standard C-18 column of the respective instrument manufacturer. For special demands, the users can bring their own chromatographic columns. The additional nanospray ion sources which can be equipped to the ESI-IMS-MS/MS or the ESI-FTICR-MS can only be used in the offline mode, i.e., without coupling to a separation technique.